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KMID : 0811719980020040419
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Korean Journal of Physiology & Pharmacology
1998 Volume.2 No. 4 p.419 ~ p.425
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Effects of Exogenous ATP on Calcium Mobilization and Cell Proliferation in C6 Glioma Cell
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Eun-Jung Lee
Seok Ho Cha/Woon Kyu Lee/Kweon-Haeng Lee/Sang-Bok Lee
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Abstract
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To clarify the effect of extracellular ATP in cultured C6 glioma cells, ATP-induced cytosolic free calcium ([Ca2+]i) mobilization and cell proliferation were investigated. ATP-induced [Ca2+]i increased in a dose-dependent manner (10?7M∼10?3M). ATP-induced [Ca2+]i increases were slightly slowed in extracellular calcium-free conditions especially in sustained phase. ATP-induced [Ca2+]i increment was also inhibited by the pretreatment of U73122, a phospholipase C (PLC) inhibitor, in a time-dependent manner. Suramin, a putative P2Y receptor antagonist, dose-dependently weakened ATP-induced [Ca2+]i mobilization. Significant increases in cell proliferation were observed at 2, 3, and 4 days after ATP was added. Stimulated cell proliferation was also observed with adenosine at days 2 and 3. This cell proliferation was significantly inhibited by the treatment with suramin. Ionomycin also stimulated cell proliferation in a concentration-dependent manner. In conclusion, we suggest that extracellular ATP stimulates C6 glioma cell proliferation via intracellular free calcium mobilization mediated by purinoceptor.
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KEYWORD
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ATP, Purinoceptor, C6 glioma cell, Cytosolic free calcium, Cell proliferation,
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